High fat diet and exercise lead to a disrupted and pathogenic DNA methylome in mouse liver.

ABSTRACT: High-fat diet consumption and sedentary lifestyle elevates risk for obesity, non-alcoholic fatty liver disease, and cancer. Exercise training conveys health benefits in populations with or without these chronic conditions. Diet and exercise regulate gene expression by mediating epigenetic mechanisms in many tissues; however, such effects are poorly documented in the liver, a central metabolic organ. To dissect the consequences of diet and exercise on the liver epigenome, we measured DNA methylation, using reduced representation bisulfite sequencing, and transcription, using RNA-seq, in mice maintained on a fast food diet with sedentary lifestyle or exercise, compared with control diet with and without exercise. Our analyses reveal that genome-wide differential DNA methylation and expressio

SUBMITTER: Zhou D 

PROVIDER: S-EPMC5270632 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature