Calcium alterations signal either to senescence or to autophagy induction in stem cells upon oxidative stress.

ABSTRACT: Intracellular calcium ([Ca²⁺]_i) has been reported to play an important role in autophagy, apoptosis and necrosis, however, a little is known about its impact in senescence. Here we investigated [Ca²⁺]_i contribution to oxidative stress-induced senescence of human endometrium-derived stem cells (hMESCs). In hMESCs sublethal H₂O₂-treatment resulted in a rapid calcium release from intracellular stores mediated by the activation of PLC/IP3/IP3R pathway. Notably, further senescence development was accompanied by persistently elevated [Ca²⁺]_i levels. In H₂O₂-treated hMESCs, [Ca²⁺]_i chelation by BAPTA-AM (BAPTA) was sufficient to prevent the expansion of the senescenc

SUBMITTER: Borodkina AV 

PROVIDER: S-EPMC5270676 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature