Analysis of embryonic development in the unsequenced axolotl: Waves of transcriptomic upheaval and stability.

ABSTRACT: The axolotl (Ambystoma mexicanum) has long been the subject of biological research, primarily owing to its outstanding regenerative capabilities. However, the gene expression programs governing its embryonic development are particularly underexplored, especially when compared to other amphibian model species. Therefore, we performed whole transcriptome polyA+ RNA sequencing experiments on 17 stages of embryonic development. As the axolotl genome is unsequenced and its gene annotation is incomplete, we built de novo transcriptome assemblies for each stage and garnered functional annotation by comparing expressed contigs with known genes in other organisms. In evaluating the number of differentially expressed genes over time, we identify three waves of substantial transcriptome upheaval each

SUBMITTER: Jiang P 

PROVIDER: S-EPMC5272911 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature