The minimal promoter region of the dense-core vesicle protein IA-2: transcriptional regulation by CREB.

ABSTRACT:

Aims

IA-2 is a transmembrane protein found in the dense-core vesicles (DCV) of neuroendocrine cells and one of the major autoantigens in type 1 diabetes. DCV are involved in the secretion of hormones (e.g., insulin) and neurotransmitters. Stimulation of pancreatic β cells with glucose upregulates the expression of IA-2 and an increase in IA-2 results in an increase in the number of DCV. Little is known, however, about the promoter region of IA-2 or the transcriptional factors that regulate the expression of this gene.

Methods

In the present study, we constructed eight deletion fragments from the upstream region of the IA-2 transcription start site and linked them to a luciferase reporter.

Results

By this approach, we have identified a short bp region (-216 to +115) th

SUBMITTER: Cai T 

PROVIDER: S-EPMC5273861 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature