A G Protein-Coupled Receptor Dimerization Interface in Human Cone Opsins.

ABSTRACT: G protein-coupled receptors (GPCRs) detect a wide variety of physical and chemical signals and transmit that information across the cellular plasma membrane. Dimerization is a proposed modulator of GPCR signaling, but the structure and stability of class A GPCR dimerization have been difficult to establish. Here we investigated the dimerization affinity and binding interface of human cone opsins, which initiate and sustain daytime color vision. Using a time-resolved fluorescence approach, we found that human red cone opsin exhibits a strong propensity for dimerization, whereas the green and blue cone opsins do not. Through mutagenesis experiments, we identified a dimerization interface in the fifth transmembrane helix of human red cone opsin involving amino acids I230, A233, and M236. Insi

SUBMITTER: Jastrzebska B 

PROVIDER: S-EPMC5274527 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature