Reduced Osteoarthritis Severity in Aged Mice With Deletion of Macrophage Migration Inhibitory Factor.

ABSTRACT:

Objective

Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that is elevated in the serum and synovial fluid of patients with osteoarthritis (OA). This study was undertaken to investigate the potential role of MIF in OA in human joint tissues and in vivo in mice with age-related and surgically induced OA.

Methods

MIF in conditioned media from human chondrocytes and meniscal cells and from cartilage explants was measured by enzyme-linked immunosorbent assay. The severity of OA was analyzed histologically in male wild-type and MIF^-/- mice at 12 and 22 months of age and following destabilization of the medial meniscus (DMM) surgery in 12-week-old MIF^-/- mice as well as in wild-type mice treated with a neutralizing MIF antibody. Syn

SUBMITTER: Rowe MA 

PROVIDER: S-EPMC5274570 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature