Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems.

ABSTRACT: Combined measurement of diverse molecular and anatomical traits that span multiple levels remains a major challenge in biology. Here, we introduce a simple method that enables proteomic imaging for scalable, integrated, high-dimensional phenotyping of both animal tissues and human clinical samples. This method, termed SWITCH, uniformly secures tissue architecture, native biomolecules, and antigenicity across an entire system by synchronizing the tissue preservation reaction. The heat- and chemical-resistant nature of the resulting framework permits multiple rounds (>20) of relabeling. We have performed 22 rounds of labeling of a single tissue with precise co-registration of multiple datasets. Furthermore, SWITCH synchronizes labeling reactions to improve probe penetration depth and uniform

SUBMITTER: Murray E 

PROVIDER: S-EPMC5275966 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature