Defective natural killer cell activity in a mouse model of eczema herpeticum.

ABSTRACT:

Background

Patients with atopic dermatitis (AD) are susceptible to several viruses, including herpes simplex virus (HSV). Some patients experience 1 or more episodes of a severe skin infection caused by HSV termed eczema herpeticum (EH). There are numerous mouse models of AD, but no established model exists for EH.

Objective

We sought to establish and characterize a mouse model of EH.

Methods

We infected AD-like skin lesions with HSV1 to induce severe skin lesions in a dermatitis-prone mouse strain of NC/Nga. Gene expression was investigated by using a microarray and quantitative PCR; antibody titers were measured by means of ELISA; and natural killer (NK) cell, cytotoxic T-cell, regulatory T-cell, and follicular helper T-cell populations were evaluated by using flow

SUBMITTER: Kawakami Y 

PROVIDER: S-EPMC5276800 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature