Synaptotagmin 2 Is the Fast Ca²⁺ Sensor at a Central Inhibitory Synapse.

ABSTRACT: GABAergic synapses in brain circuits generate inhibitory output signals with submillisecond latency and temporal precision. Whether the molecular identity of the release sensor contributes to these signaling properties remains unclear. Here, we examined the Ca²⁺ sensor of exocytosis at GABAergic basket cell (BC) to Purkinje cell (PC) synapses in cerebellum. Immunolabeling suggested that BC terminals selectively expressed synaptotagmin 2 (Syt2), whereas synaptotagmin 1 (Syt1) was enriched in excitatory terminals. Genetic elimination of Syt2 reduced action potential-evoked release to ∼10%, identifying Syt2 as the major Ca²⁺ sensor at BC-PC synapses. Differential adenovirus-mediated rescue revealed that Syt2 triggered release with shorter latency and higher temporal prec

SUBMITTER: Chen C 

PROVIDER: S-EPMC5276807 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature