Superoxide Generation and Its Involvement in the Growth of Mycobacterium smegmatis.

ABSTRACT: Superoxide generation is inevitable in aerobic organisms, most of which have developed mechanisms to detoxify superoxides. However, its significance has not been clearly understood in mycobacteria. This study demonstrates that NADH oxidase is the major source of superoxide in Mycobacterium smegmatis and elucidates the involvement of superoxide in M. smegmatis growth. The maximum inhibition of superoxide generation was observed in the presence of diphenyleneiodonium chloride (DPI), an NADH oxidase inhibitor, compared to other standard inhibitors. After incubation for 24 h, the number of colony forming units (CFUs) was reduced by 6.8 log₁₀ compared to the untreated culture. The inhibitory effect of DPI on M. smegmatis was reversed when the same culture was sup

SUBMITTER: Yeware AM 

PROVIDER: S-EPMC5276846 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature