Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein.

ABSTRACT:

Background and objectives

Toxoplasma gondii is an obligatory intracellular parasite which causes severe diseases in the fetus of pregnant women and immunocopmromised patients. Serological tests based on recombinant protein are one of the main diagnosis methods for the detection of specific antibodies in serum samples. Dense granule antigenic proteins derived from T. gondii (TgGRAs) are potential antigens for the development of diagnostic tools.

Materials and methods

DNA was extracted from T. gondii (RH-strain) tachyzoites and PCR reaction was done using corresponding primers for GRA5 antigen. The PCR product was purified and ligated into pTG19-t vector and then subcloned into XhoI and BamHI digested pGEX6p-1 expression vector. Recombinant p

SUBMITTER: Arab-Mazar Z 

PROVIDER: S-EPMC5277603 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature