Synchronized purification and immobilization of his-tagged β-glucosidase via Fe₃O₄/PMG core/shell magnetic nanoparticles.

ABSTRACT: In this paper, an efficient and convenient Fe₃O₄/PMG/IDA-Ni²⁺ nanoparticles that applied to purify and immobilize his-tagged β-glucosidase was synthesized, in which, Fe₃O₄/PMG (poly (N, N'-methylenebisacrylamide-co-glycidyl methacrylate) core/shell microspheres were synthesized firstly using distillation-precipitation polymerization, then iminodiacetic acid (IDA) was used to open epoxy rings on the shell of microspheres to the combination of Ni²⁺. The gene of β-glucosidase that was from Coptotermes formosanus Shiraki was amplified, cloned into the expression vector pET28a with an N-terminal His-tag, and expressed in E.coli BL21. The nanoparticles showed the same purification efficiency as commercial nickel column which was a

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC5278371 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature