Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D.

ABSTRACT: We describe and share a device, methodology and image analysis algorithms, which allow up to 66 spheroids to be arranged into a gel-based array directly from a culture plate for downstream processing and analysis. Compared to processing individual samples, the technique uses 11-fold less reagents, saves time and enables automated imaging. To illustrate the power of the technology, we showcase applications of the methodology for investigating 3D spheroid morphology and marker expression and for in vitro safety and efficacy screens. First, spheroid arrays of 11 cell-lines were rapidly assessed for differences in spheroid morphology. Second, highly-positive (SOX-2), moderately-positive (Ki-67) and weakly-positive (βIII-tubulin) protein targets were detected and quantified. Third, the arrays e

SUBMITTER: Ivanov DP 

PROVIDER: S-EPMC5278392 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature