Calsequestrin depolymerizes when calcium is depleted in the sarcoplasmic reticulum of working muscle.

ABSTRACT: Calsequestrin, the only known protein with cyclical storage and supply of calcium as main role, is proposed to have other functions, which remain unproven. Voluntary movement and the heart beat require this calcium flow to be massive and fast. How does calsequestrin do it? To bind large amounts of calcium in vitro, calsequestrin must polymerize and then depolymerize to release it. Does this rule apply inside the sarcoplasmic reticulum (SR) of a working cell? We answered using fluorescently tagged calsequestrin expressed in muscles of mice. By FRAP and imaging we monitored mobility of calsequestrin as [Ca²⁺] in the SR--measured with a calsequestrin-fused biosensor--was lowered. We found that calsequestrin is polymerized within the SR at rest and that it depolymerized as [Ca²

SUBMITTER: Manno C 

PROVIDER: S-EPMC5278470 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature