Shuttling along DNA and directed processing of D-loops by RecQ helicase support quality control of homologous recombination.

ABSTRACT: Cells must continuously repair inevitable DNA damage while avoiding the deleterious consequences of imprecise repair. Distinction between legitimate and illegitimate repair processes is thought to be achieved in part through differential recognition and processing of specific noncanonical DNA structures, although the mechanistic basis of discrimination remains poorly defined. Here, we show that Escherichia coli RecQ, a central DNA recombination and repair enzyme, exhibits differential processing of DNA substrates based on their geometry and structure. Through single-molecule and ensemble biophysical experiments, we elucidate how the conserved domain architecture of RecQ supports geometry-dependent shuttling and directed processing of recombination-intermediate [displacement loop (D-loop)]

SUBMITTER: Harami GM 

PROVIDER: S-EPMC5278487 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature