Detection of Intestinal Pathogens in River, Shore, and Drinking Water in Lima, Peru.

ABSTRACT: Water quality management is an ongoing struggle for many locations worldwide. Current testing of water supplies can be time-consuming, expensive, and lack sensitivity. This study describes an alternative, easy-to-use, and inexpensive method to water sampling and testing at remote locations. This method was employed to detect a number of intestinal pathogens in various locations of Lima, Peru. A total of 34 PCR primer pairs were tested for specificity and high-yield amplification for 12 different pathogens using known DNA templates. Select primers for each pathogen were then tested for minimum detection limits of DNA. Water samples were collected from 22 locations. PCR was used to detect the presence of a pathogen, virulence factors, or differentiate between pathogenic species. In 22 water

SUBMITTER: Grothen DC 

PROVIDER: S-EPMC5278651 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature