Identification of IncA/C Plasmid Replication and Maintenance Genes and Development of a Plasmid Multilocus Sequence Typing Scheme.

ABSTRACT: Plasmids of incompatibility group A/C (IncA/C) are becoming increasingly prevalent within pathogenic Enterobacteriaceae They are associated with the dissemination of multiple clinically relevant resistance genes, including bla_CMY and bla_NDM Current typing methods for IncA/C plasmids offer limited resolution. In this study, we present the complete sequence of a bla_NDM-1-positive IncA/C plasmid, pMS6198A, isolated from a multidrug-resistant uropathogenic Escherichia coli strain. Hypersaturated transposon mutagenesis, coupled with transposon-directed insertion site sequencing (TraDIS), was employed to identify conserved genetic elements required for replication and maintenance of pMS6198A. Our analysis of TraDIS data identified roles for the replicon, includin

SUBMITTER: Hancock SJ 

PROVIDER: S-EPMC5278728 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature