Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering.

ABSTRACT: Targeted genome editing has become a powerful genetic tool for studying gene function or for modifying genomes by correcting defective genes or introducing genes. A variety of reagents have been developed in recent years that can generate targeted double-stranded DNA cuts which can be repaired by the error-prone, non-homologous end joining repair system or via the homologous recombination-based double-strand break repair pathway provided a suitable template is available. These genome editing reagents require components for recognizing a specific DNA target site and for DNA-cleavage that generates the double-stranded break. In order to reduce potential toxic effects of genome editing reagents, it might be desirable to control the in vitro or in vivo activity of these reagents by incorporati

SUBMITTER: Guha TK 

PROVIDER: S-EPMC5279741 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature