Standardization of microparticle enumeration across different flow cytometry platforms: results of a multicenter collaborative workshop.

ABSTRACT: Essentials The clinical enumeration of microparticles (MPs) is hampered by a lack of standardization. A new strategy to standardize MP counts by flow cytometry was evaluated in a multicenter study. No difference was found between instruments using forward or side scatter as the trigger parameter. This study demonstrated that beads can be used as a standardization tool for MPs. Click to hear the ISTH Academy's webinar on microvesicles SUMMARY: Background Microparticles (MPs) are extracellular vesicles resulting from the budding of cellular membranes that have a high potential as emergent biomarkers; however, their clinical relevance is hampered by methodological enumeration concerns and a lack of standardization. Flow cytometry (FCM) remains the most commonly used technique with the best ca

SUBMITTER: Cointe S 

PROVIDER: S-EPMC5280151 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature