Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome.

ABSTRACT:

Background

Through functional screening of a fosmid library, generated from a phytopathogen-suppressive soil metagenome, the novel antifungal chitinase-named Chi18H8 and belonging to family 18 glycosyl hydrolases-was previously discovered. The initial extremely low yield of Chi18H8 recombinant production and purification from Escherichia coli cells (21 μg/g cell) limited its characterization, thus preventing further investigation on its biotechnological potential.

Results

We report on how we succeeded in producing hundreds of milligrams of pure and biologically active Chi18H8 by developing and scaling up to a high-yielding, 30 L bioreactor process, based on a novel method of mild solubilization of E. coli inclusion bodies in lactic acid aqueous solution, coupled with a singl

SUBMITTER: Berini F 

PROVIDER: S-EPMC5282697 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature