Quercetin-induced miR-200b-3p regulates the mode of self-renewing divisions in pancreatic cancer.

ABSTRACT:

Background

Cancer stem cells are suggested to contribute to the extremely poor prognosis of pancreatic ductal adenocarcinoma and dysregulation of symmetric and asymmetric stem cell division may be involved. Anticancer benefits of phytochemicals like the polyphenol quercetin, present in many fruits, nuts and vegetables, could be expedited by microRNAs, which orchestrate cell-fate decisions and tissue homeostasis. The mechanisms regulating the division mode of cancer stem cells in relation to phytochemical-induced microRNAs are poorly understood.

Methods

Patient-derived pancreas tissue and 3 established pancreatic cancer cell lines were examined by immunofluorescence and time-lapse microscopy, microRNA microarray analysis, bioinformatics and computational analysis, qRT-PCR, We

SUBMITTER: Nwaeburu CC 

PROVIDER: S-EPMC5282715 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature