Improved annotation with de novo transcriptome assembly in four social amoeba species.

ABSTRACT:

Background

Annotation of gene models and transcripts is a fundamental step in genome sequencing projects. Often this is performed with automated prediction pipelines, which can miss complex and atypical genes or transcripts. RNA sequencing (RNA-seq) data can aid the annotation with empirical data. Here we present de novo transcriptome assemblies generated from RNA-seq data in four Dictyostelid species: D. discoideum, P. pallidum, D. fasciculatum and D. lacteum. The assemblies were incorporated with existing gene models to determine corrections and improvement on a whole-genome scale. This is the first time this has been performed in these eukaryotic species.

Results

An initial de novo transcriptome assembly was generated by Trinity for each species and then refined with Prog

SUBMITTER: Singh R 

PROVIDER: S-EPMC5282741 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature