RAD-Seq analysis of typical and minor Citrus accessions, including Bhutanese varieties.

ABSTRACT: We analyzed the reduced-representation genome sequences of Citrus species by double-digest restriction site-associated DNA sequencing (ddRAD-Seq) using 44 accessions, including typical and minor accessions, such as Bhutanese varieties. The results of this analysis using typical accessions were consistent with previous reports that citron, papeda, pummelo, and mandarin are ancestral species, and that most Citrus species are derivatives or hybrids of these four species. Citrus varieties often reproduce asexually and heterozygosity is highly conserved within each variety. Because this approach could readily detect conservation of heterozygosity, it was able to discriminate citrus varieties such as satsuma mandarin from closely related species. Thus, this method provides a

SUBMITTER: Penjor T 

PROVIDER: S-EPMC5282754 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature