Clearance Kinetics and Clearance Routes of Molecules From the Suprachoroidal Space After Microneedle Injection.

ABSTRACT:

Purpose

To determine clearance kinetics and routes of clearance of molecules from the suprachoroidal space (SCS) of live New Zealand White rabbits.

Methods

Suprachoroidal space collapse rate and pressure changes after microneedle injection into SCS were determined. Fluorescent fundus images were acquired to determine clearance rates of molecules ranging in size from 332 Da to 2 MDa. Microneedle injections of fluorescein were performed, and samples were taken from various sites over time to determine amount of fluorescein exiting the eye. Clearance transport was modeled theoretically and compared with experimental data.

Results

After injection, pressures in SCS and vitreous humor spiked and returned to baseline within 20 minutes; there was no difference between these t

SUBMITTER: Chiang B 

PROVIDER: S-EPMC5283080 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature