Proteomic Analysis of the Bovine and Human Ciliary Zonule.

ABSTRACT:

Purpose

The zonule of Zinn (ciliary zonule) is a system of fibers that centers the crystalline lens on the optical axis of the eye. Mutations in zonule components underlie syndromic conditions associated with a broad range of ocular pathologies, including microspherophakia and ectopia lentis. Here, we used HPLC-mass spectrometry to determine the molecular composition of the zonule.

Methods

Tryptic digests of human and bovine zonular samples were analyzed by HPLC-mass spectrometry. The distribution of selected components was confirmed by immunofluorescence confocal microscopy. In bovine samples, the composition of the equatorial zonule was compared to that of the hyaloid zonule and vitreous humor.

Results

The 52 proteins common to the zonules of both species accounted

SUBMITTER: De Maria A 

PROVIDER: S-EPMC5283081 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature