MPLEx: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling.

ABSTRACT: The continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. Molecular characterization of infectious samples outside of appropriate biosafety containment can take place only subsequent to pathogen inactivation. Herein, we describe a modified Folch extraction using chloroform/methanol that facilitates the molecular characterization of infectious samples by enabling simultaneous pathogen inactivation and extraction of proteins, metabolites, and lipids for subsequent mass spectrometry-based multi-omics measurements. This single-sample metabolite, protein and lipid extraction (MPLEx) method resu

SUBMITTER: Burnum-Johnson KE 

PROVIDER: S-EPMC5283721 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature