Prostaglandin E₂ Activates YAP and a Positive-Signaling Loop to Promote Colon Regeneration After Colitis but Also Carcinogenesis in Mice.

ABSTRACT:

Background & aims

Prostaglandin E₂ (PGE₂) is mediator of inflammation that regulates tissue regeneration, but its continual activation has been associated with carcinogenesis. Little is known about factors in the PGE₂ signaling pathway that contribute to tumor formation. We investigated whether yes-associated protein 1 (YAP1), a transcriptional co-activator in the Hippo signaling pathway, mediates PGE₂ function.

Methods

DLD-1 and SW480 colon cancer cell lines were transfected with vectors expressing transgenes or small hairpin RNAs and incubated with recombinant PGE₂, with or without pharmacologic inhibitors of signaling proteins, and analyzed by immunoblot, immunofluorescence, quantitative reverse-transcription polymera

SUBMITTER: Kim HB 

PROVIDER: S-EPMC5285392 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature