Hippo kinases maintain polarity during directional cell migration in Caenorhabditis elegans.

ABSTRACT: Precise positioning of cells is crucial for metazoan development. Despite immense progress in the elucidation of the attractive cues of cell migration, the repulsive mechanisms that prevent the formation of secondary leading edges remain less investigated. Here, we demonstrate that Caenorhabditis elegans Hippo kinases promote cell migration along the anterior-posterior body axis via the inhibition of dorsal-ventral (DV) migration. Ectopic DV polarization was also demonstrated in gain-of-function mutant animals for C. elegans RhoG MIG-2. We identified serine 139 of MIG-2 as a novel conserved Hippo kinase phosphorylation site and demonstrated that purified Hippo kinases directly phosphorylate MIG-2^S139 Live imaging analysis of genome-edited animals indicates that MIG-2^S139

SUBMITTER: Feng G 

PROVIDER: S-EPMC5286363 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature