Distinct prostate cancer-related mRNA cargo in extracellular vesicle subsets from prostate cell lines.

ABSTRACT:

Background

Multiple types of extracellular vesicles (EVs), including microvesicles (MVs) and exosomes (EXOs), are released by all cells constituting part of the cellular EV secretome. The bioactive cargo of EVs can be shuffled between cells and consists of lipids, metabolites, proteins, and nucleic acids, including multiple RNA species from non-coding RNAs to messenger RNAs (mRNAs). In this study, we hypothesized that the mRNA cargo of EVs could differ based on the EV cellular origin and subpopulation analyzed.

Methods

We isolated MVs and EXOs from PC-3 and LNCaP prostate cancer cells by differential centrifugation and compared them to EVs derived from the benign PNT2 prostate cells. The relative mRNA levels of 84 prostate cancer-related genes were investigated and validated

SUBMITTER: Lazaro-Ibanez E 

PROVIDER: S-EPMC5286827 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature