Characterization of hundreds of regulatory landscapes in developing limbs reveals two regimes of chromatin folding.

ABSTRACT: Complex regulatory landscapes control the pleiotropic transcriptional activities of developmental genes. For most genes, the number, location, and dynamics of their associated regulatory elements are unknown. In this work, we characterized the three-dimensional chromatin microarchitecture and regulatory landscape of 446 limb-associated gene loci in mouse using Capture-C, ChIP-seq, and RNA-seq in forelimb, hindlimb at three developmental stages, and midbrain. The fine mapping of chromatin interactions revealed a strong preference for functional genomic regions such as repressed or active domains. By combining chromatin marks and interaction peaks, we annotated more than 1000 putative limb enhancers and their associated genes. Moreover, the analysis of chromatin interactions revealed two reg

SUBMITTER: Andrey G 

PROVIDER: S-EPMC5287228 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature