Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets.

ABSTRACT: Cytokinesis involves temporally and spatially coordinated action of the cell cycle and cytoskeletal and membrane systems to achieve separation of daughter cells. To dissect cytokinesis mechanisms it would be useful to have a complete catalog of the proteins involved, and small molecule tools for specifically inhibiting them with tight temporal control. Finding active small molecules by cell-based screening entails the difficult step of identifying their targets. We performed parallel chemical genetic and genome-wide RNA interference screens in Drosophila cells, identifying 50 small molecule inhibitors of cytokinesis and 214 genes important for cytokinesis, including a new protein in the Aurora B pathway (Borr). By comparing small molecule and RNAi phenotypes, we identified a small molecule

SUBMITTER: Eggert US 

PROVIDER: S-EPMC528723 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature