Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR.

ABSTRACT: Along with their cognate acyl-homoserine lactone signals, the quorum sensing regulators LasR and RhlR control the expression of hundreds of genes in the opportunistic human pathogen Pseudomonas aeruginosa. This extensive, overlapping regulatory network affords the opportunity to systematically investigate the sequence requirements and specificity determinants of large families of target promoters. Many of the P. aeruginosa quorum-controlled genes possess conserved palindromic promoter elements predicted to be binding sites for either one or both transcriptional regulators, but biochemical proof has not been reported. We have purified native LasR and characterized binding to various quorum-controlled promoters in vitro. Purified LasR was a dimer in solution that irreversibly bound two molec

SUBMITTER: Schuster M 

PROVIDER: S-EPMC528741 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature