RNAi-Based Functional Genomics Identifies New Virulence Determinants in Mucormycosis.

ABSTRACT: Mucorales are an emerging group of human pathogens that are responsible for the lethal disease mucormycosis. Unfortunately, functional studies on the genetic factors behind the virulence of these organisms are hampered by their limited genetic tractability, since they are reluctant to classical genetic tools like transposable elements or gene mapping. Here, we describe an RNAi-based functional genomic platform that allows the identification of new virulence factors through a forward genetic approach firstly described in Mucorales. This platform contains a whole-genome collection of Mucor circinelloides silenced transformants that presented a broad assortment of phenotypes related to the main physiological processes in fungi, including virulence, hyphae morphology, mycelial and yeast growth

SUBMITTER: Trieu TA 

PROVIDER: S-EPMC5287474 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature