The mitotic tensegrity guardian tau protects mammary epithelia from katanin-like1-induced aneuploidy.

ABSTRACT: The microtubule associated-protein tau has been identified as an effective positive prognostic indicator in breast cancer. To explore the physiological function of tau in early carcinogenesis, endogenous tau was knocked down in primary cultured human mammary epithelial cells. This resulted in chromosome-bridging during anaphase followed by micronucleation, both of which were suppressed by a further katanin-like1 knockdown. We also detected that the exogenously expressed katanin-like1 induction of cellular transformation is prevented by exogenous tau in rat fibroblasts. The mutant katanin-like1 (L123V) identified in breast cancer showed an increase in this transformation capacity as well as microtubule severing activity resistant to tau. The tau knockdown resulted in a loss of the kinetocho

SUBMITTER: Sudo H 

PROVIDER: S-EPMC5288216 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature