Comparative transcriptome analysis reveals a regulatory network of microRNA-29b during mouse early embryonic development.

ABSTRACT: MicroRNAs are endogenous ~22 nt RNAs that regulate gene expression by translational inhibition and mRNA destabilization. MicroRNA-29b (miR-29b) is essential for progression of mouse embryos past preimplantation development; however, details of the underlying regulatory network remain to be elucidated. Here, we used RNA sequencing to identify changes in the transcriptome of mouse embryos in response to miR-29b inhibition. Morula-stage embryos that had been subject to miR-29b inhibition throughout preimplantation development exhibited significant expression changes in 870 genes compared with controls. Among 405 genes that were downregulated, 30 genes encoded factors with known essential function during early embryonic development, including the pluripotent stem cell factor Nanog. We identifi

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5288220 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature