Coexistence of bla_OXA-48 and Truncated bla_NDM-1 on Different Plasmids in a Klebsiella pneumoniae Isolate in China.

ABSTRACT: Objectives: To describe the genetic environment, transferability, and antibiotic susceptibility of one clinical Klebsiella pneumoniae isolate harboring both bla_OXA-48 and bla_NDM-1 on different plasmids from a Chinese hospital. Methods: The isolate was subjected to antimicrobial susceptibility testing and multilocus sequence typing using Etest and PCR. The plasmids harboring bla_OXA-48 and bla_NDM-1 were analyzed through conjugation experiments, S1-nuclease pulsed-field gel electrophoresis, and hybridization with specific probes. Plasmid DNA was sequenced using Pacbio RS II and annotated using RAST. Results:K. pneumoniae RJ119, carrying both bla_OXA-48 and bla

SUBMITTER: Xie L 

PROVIDER: S-EPMC5288367 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature