FlrA Represses Transcription of the Biofilm-Associated bpfA Operon in Shewanella putrefaciens.

ABSTRACT: Manipulation of biofilm formation in Shewanella is beneficial for application to industrial and environmental biotechnology. BpfA is an adhesin largely responsible for biofilm formation in many Shewanella species. However, the mechanism underlying BpfA production and the resulting biofilm remains vaguely understood. We previously described the finding that BpfA expression is enhanced by DosD, an oxygen-stimulated diguanylate cyclase, under aerobic growth. In the present work, we identify FlrA as a critical transcription regulator of the bpfA operon in Shewanella putrefaciens CN32 by transposon mutagenesis. FlrA acted as a repressor of the operon promoter by binding to two boxes overlapping the -10 and -35 sites recognized by σ70 DosD regulation of the expression of the bpfA operon was medi

SUBMITTER: Cheng YY 

PROVIDER: S-EPMC5288817 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature