The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

ABSTRACT: The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and hum

SUBMITTER: Gerhard DS 

PROVIDER: S-EPMC528928 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature