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Genes required for Drosophila nervous system development identified by RNA interference.

ABSTRACT: RNA interference was used to screen 3,314 Drosophila double-stranded RNAs, corresponding to approximately 25% of Drosophila genes, for genes that affect the development of the embryonic nervous system. RNA-interference-mediated gene silencing in Drosophila embryos resulted in loss-of-function mutant phenotypes for 43 genes, which is 1.3% of the genes that were screened. We found 18 genes that were not known previously to affect the development of the nervous system. The functions of some of the genes are unknown. Other genes encode protein kinases, transcription factors, and signaling proteins, as well as proteins with other functions.

SUBMITTER: Ivanov AI

PROVIDER: S-EPMC528945 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature

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Genes required for Drosophila nervous system development identified by RNA interference.

Ivanov Andrej I AI Rovescalli Alessandra C AC Pozzi Paola P Yoo Siuk S Mozer Brian B Li Hsi-Ping HP Yu Shu-Hua SH Higashida Haruhiro H Guo Vicky V Spencer Michael M Nirenberg Marshall M

Proceedings of the National Academy of Sciences of the United States of America 20041108 46

RNA interference was used to screen 3,314 Drosophila double-stranded RNAs, corresponding to approximately 25% of Drosophila genes, for genes that affect the development of the embryonic nervous system. RNA-interference-mediated gene silencing in Drosophila embryos resulted in loss-of-function mutant phenotypes for 43 genes, which is 1.3% of the genes that were screened. We found 18 genes that were not known previously to affect the development of the nervous system. The functions of some of the ...[more]

PMID: 15534205

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