Transition state and encounter complex for fast association of cytochrome c2 with bacterial reaction center.

ABSTRACT: Electrostatic interactions strongly enhance the electron transfer reaction between cytochrome (Cyt) c(2) and reaction center (RC) from photosynthetic bacteria, yielding a second-order rate constant, k(2) approximately 10(9) s(-1).M(-1), close to the diffusion limit. The proposed mechanism involves an encounter complex (EC) stabilized by electrostatic interactions, followed by a transition state (TS), leading to the bound complex active in electron transfer. The effect of electrostatic interactions was previously studied by Tetreault et al. [Tetreault, M., Cusanovich, M., Meyer, T., Axelrod, H. & Okamura, M. Y. (2002) Biochemistry 41, 5807-5815] by measuring k(2) for RC and Cyt molecules with modified charged residues at the binding interface. The present work is a computational analysis of

SUBMITTER: Miyashita O 

PROVIDER: S-EPMC528947 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature