Identification of the early and late responder genes during the generation of induced pluripotent stem cells from mouse fibroblasts.

ABSTRACT:

Background

The generation of induced pluripotent stem cell (iPSC), a substitute for embryonic stem cell (ESC), requires the proper orchestration of a transcription program at the chromatin level. Our recent approach for the induction of pluripotent stem cells from fibroblasts using protein extracts from mouse ESCs could overcome the potential tumorigenicity risks associated with random retroviral integration. Here, we examine the epigenetic modifications and the transcriptome of two types of iPSC and of partially reprogrammed iPSCs (iPSCp) generated independently from adult cardiac and skin fibroblasts to assess any perturbations of the transcription program during reprogramming.

Results

The comparative dissection of the transcription profiles and histone modification patter

SUBMITTER: Park J 

PROVIDER: S-EPMC5289558 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature