Pathogen-specific deep sequence-coupled biopanning: A method for surveying human antibody responses.

ABSTRACT: Identifying the targets of antibody responses during infection is important for designing vaccines, developing diagnostic and prognostic tools, and understanding pathogenesis. We developed a novel deep sequence-coupled biopanning approach capable of identifying the protein epitopes of antibodies present in human polyclonal serum. Here, we report the adaptation of this approach for the identification of pathogen-specific epitopes recognized by antibodies elicited during acute infection. As a proof-of-principle, we applied this approach to assessing antibodies to Dengue virus (DENV). Using a panel of sera from patients with acute secondary DENV infection, we panned a DENV antigen fragment library displayed on the surface of bacteriophage MS2 virus-like particles and characterized the populat

SUBMITTER: Frietze KM 

PROVIDER: S-EPMC5289605 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature