Anaerobically Grown Escherichia coli Has an Enhanced Mutation Rate and Distinct Mutational Spectra.

ABSTRACT: Oxidative stress is a major cause of mutation but little is known about how growth in the absence of oxygen impacts the rate and spectrum of mutations. We employed long-term mutation accumulation experiments to directly measure the rates and spectra of spontaneous mutation events in Escherichia coli populations propagated under aerobic and anaerobic conditions. To detect mutations, whole genome sequencing was coupled with methods of analysis sufficient to identify a broad range of mutational classes, including structural variants (SVs) generated by movement of repetitive elements. The anaerobically grown populations displayed a mutation rate nearly twice that of the aerobic populations, showed distinct asymmetric mutational strand biases, and greater insertion element activity. Consistent

SUBMITTER: Shewaramani S 

PROVIDER: S-EPMC5289635 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature