Feeding-Related Traits Are Affected by Dosage of the foraging Gene in Drosophila melanogaster.

ABSTRACT: Nutrient acquisition and energy storage are critical parts of achieving metabolic homeostasis. The foraging gene in Drosophila melanogaster has previously been implicated in multiple feeding-related and metabolic traits. Before foraging's functions can be further dissected, we need a precise genetic null mutant to definitively map its amorphic phenotypes. We used homologous recombination to precisely delete foraging, generating the for⁰ null allele, and used recombineering to reintegrate a full copy of the gene, generating the {for^BAC} rescue allele. We show that a total loss of foraging expression in larvae results in reduced larval path length and food intake behavior, while conversely showing an increase in triglyceride levels. Furthermore, varying foraging gene do

SUBMITTER: Allen AM 

PROVIDER: S-EPMC5289850 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature