IL-33 Drives Augmented Responses to Ozone in Obese Mice.

ABSTRACT:

Background

Ozone increases IL-33 in the lungs, and obesity augments the pulmonary effects of acute ozone exposure.

Objectives

We assessed the role of IL-33 in the augmented effects of ozone observed in obese mice.

Methods

Lean wildtype and obese db/db mice were pretreated with antibodies blocking the IL-33 receptor, ST2, and then exposed to ozone (2 ppm for 3 hr). Airway responsiveness was assessed, bronchoalveolar lavage (BAL) was performed, and lung cells harvested for flow cytometry 24 hr later. Effects of ozone were also assessed in obese and lean mice deficient in γδ T cells and their wildtype controls.

Results and discussion

Ozone caused greater increases in BAL IL-33, neutrophils, and airway responsiveness in obese than lean mice. Anti-ST2 reduced ozone-

SUBMITTER: Mathews JA 

PROVIDER: S-EPMC5289908 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature