Role of TGFBIp in Wound Healing and Mucin Expression in Corneal Epithelial Cells.

ABSTRACT:

Purpose

Transforming growth factor-β-induced protein (TGFBIp) is highly expressed in the cornea, and mutant TGFBIp induces corneal diseases. However, the function of TGFBIp in cornea epithelium is not fully investigated. Here, we tested the importance of TGFBIp in regulation of gene expression and corneal epithelial cell (CEC) activity.

Materials and methods

The effect of TGFBIp on CEC activity was analyzed by cell migration, adhesion, proliferation and wound healing assay. Analysis of gene expression was examined by western blot and quantitative reverse transcription PCR.

Results

The results demonstrated that TGFBIp increased adhesion, migration, proliferation, and wound healing of CECs. Analysis of gene expression presented that TGFBIp-stimulated CECs exhibited incr

SUBMITTER: Maeng YS 

PROVIDER: S-EPMC5290024 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature