Nuclear Localized LSR: A Novel Regulator of Breast Cancer Behavior and Tumorigenesis.

ABSTRACT: Lipolysis-stimulated lipoprotein receptor (LSR) has been found in the plasma membrane and is believed to function in lipoprotein endocytosis and tight junctions. Given the impact of cellular metabolism and junction signaling pathways on tumor phenotypes and patient outcome, it is important to understand how LSR cellular localization mediates its functions. We conducted localization studies, evaluated DNA binding, and examined the effects of nuclear LSR in cells, xenografts, and clinical specimens. We found LSR within the membrane, cytoplasm, and the nucleus of breast cancer cells representing multiple intrinsic subtypes. Chromatin immunoprecipitation (ChIP) showed direct binding of LSR to DNA, and sequence analysis identified putative functional motifs and post-translational modifications

SUBMITTER: Reaves DK 

PROVIDER: S-EPMC5290211 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature