Transcriptomic and anatomic parcellation of 5-HT_3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing.

ABSTRACT: Cortical GABAergic interneurons constitute a highly diverse population of inhibitory neurons that are key regulators of cortical microcircuit function. An important and heterogeneous group of cortical interneurons specifically expresses the serotonin receptor 3A (5-HT_3AR) but how this diversity emerges during development is poorly understood. Here we use single-cell transcriptomics to identify gene expression patterns operating in Htr3a-GFP+ interneurons during early steps of cortical circuit assembly. We identify three main molecular types of Htr3a-GFP+ interneurons, each displaying distinct developmental dynamics of gene expression. The transcription factor Meis2 is specifically enriched in a type of Htr3a-GFP+ interneurons largely confined to the cortical white matter. These

SUBMITTER: Frazer S 

PROVIDER: S-EPMC5290279 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature