Dual origins of the intracellular circadian calcium rhythm in the suprachiasmatic nucleus.

ABSTRACT: In mammals, the master circadian clock is located in the suprachiasmatic nucleus (SCN), where most neurons show circadian rhythms of intracellular Ca²⁺ levels. However, the origin of these Ca²⁺ rhythms remains largely unknown. In this study, we successfully monitored the intracellular circadian Ca²⁺ rhythms together with the circadian PER2 and firing rhythms in a single SCN slice ex vivo, which enabled us to explore the origins. The phase relation between the circadian PER2 and Ca²⁺ rhythms, but not between the circadian PER2 and firing rhythms, was significantly altered in Cry1/Cry2 double knockout mice, which display a loss of intercellular synchronization in the SCN. In addition, in Cry1/Cry2 double knockout mice, circadian Ca²⁺ rhy

SUBMITTER: Enoki R 

PROVIDER: S-EPMC5290527 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature