Characterizing Protein Glycosylation through On-Chip Glycan Modification and Probing.

ABSTRACT: Glycans are critical to protein biology and are useful as disease biomarkers. Many studies of glycans rely on clinical specimens, but the low amount of sample available for some specimens limits the experimental options. Here we present a method to obtain information about protein glycosylation using a minimal amount of protein. We treat proteins that were captured or directly spotted in small microarrays (2.2 mm × 2.2 mm) with exoglycosidases to successively expose underlying features, and then we probe the native or exposed features using a panel of lectins or glycan-binding reagents. We developed an algorithm to interpret the data and provide predictions about the glycan motifs that are present in the sample. We demonstrated the efficacy of the method to characterize differences between

SUBMITTER: Reatini BS 

PROVIDER: S-EPMC5290727 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature